DISSABS / 2006:175

AN

    2006:175  DISSABS   Order Number: AAIC821247 (not available for sale by
    UMI)

TI

    Hormone-sensitive lipase: New roles for an old enzyme

AU

    Hansson, Ola Axel [Ph.D.]

CS

    Lunds Universitet (Sweden) (0899)

SO

    Dissertation Abstracts International, (2005) Vol. 66, No. 4C, p. 766.
    Order No.: AAIC821247 (not available for sale by UMI). 147 pages. 
    ISBN: 91-85439-76-2.

DT

    Dissertation

FS

    DAI

LA

    English

ED

    Entered STN: 20060126
    Last Updated on STN: 20060126

AB

         Hormone-sensitive lipase (HSL) has a unique ability to
    hydrolyze a large panel of substrates including acylglycerols, cholesteryl
    esters and retinyl esters. HSL is potentially a new drug target for the
    treatment of obesity and type II diabetes. However, as HSL is not only
    expressed in white adipose tissue (WAT), but also plays significant roles
    in other tissues, it is of importance to better understand the different
    functions of this enzyme in the body.
         In this thesis the aim has been to study the consequences of a
    targeted disruption of the HSL gene in the mouse with focus on the
    effects in skeletal muscle and WAT.
         Expressional and functional analyses of soleus muscle of HSL null
    mice suggests an important role of HSL in skeletal muscle metabolism as
    the absence of HSL leads to increased glycogen utilization and an
    increased amount of lipid droplets, which presumably reflects a metabolic
    switch from lipid to carbohydrate metabolism.
         A fibre type transformation from slow twitch oxidative fibres to an
    enrichment of fast twitch glycolytic fibres in soleus muscle is also
    suggested.
         Using a proteomic approach a local inflammatory response in WAT is
    demonstrated in the non-obese HSL null mouse model. New methodological
    aspects of analysing data generated by two-dimensional polyacrylamide gel
    electrophoresis are also presented. Increased energy expenditure and
    perturbation of adipogenesis are suggested reasons behind the observed
    protection against diet-induced obesity in HSL null mice. Results
    presented in this thesis suggest an important role of HSL in lipid
    signalling and adipogenesis through its action as a retinyl ester
    hydrolyze in WAT.

CC

    0379 BIOLOGY, CELL; 0487 CHEMISTRY, BIOCHEMISTRY; 0571 HEALTH SCIENCES,
    PATHOLOGY