BIOSIS / 2006:632054

AN

    2006:632054  BIOSIS

DN

    PREV200600633817

TI

    The hormone-sensitive lipase C-60G promoter polymorphism is
    associated with increased waist circumference in normal-weight subjects. 

AU

    Carlsson, E. [Reprint Author]; Johansson, L. E.; Strom, K.; Hoffstedt, J.;
    Groop, L.; Holm, C.; Ridderstrale, M.

CS

    Lund Univ, Malmo Univ Hosp, Dept Clin Sci Diabet and Endocrinol, S-20502
    Malmo, Sweden
    emma.carlsson@med.lu.se

SO

    International Journal of Obesity, (SEP 2006) Vol. 30, No. 9, pp.
    1442-1448. 
    CODEN: IJOBDP. ISSN: 0307-0565. 

DT

    Article

LA

    English

ED

    Entered STN: 22 Nov 2006
    Last Updated on STN: 22 Nov 2006

AB

    Objective: Hormone-sensitive lipase (HSL) is a key enzyme in the
    mobilization of fatty acids from triglyceride stores in adipocytes.  The
    aim of the present study was to investigate the role of the HSL gene
    promoter variant C-60G, a polymorphism which previously has been
    associated with reduced promoter activity in vitro, in obesity and type 2
    diabetes.Design: We genotyped two materials consisting of obese subjects
    and non-obese controls, one material with offspring-parents trios, where
    the offspring was abdominally obese and one material with trios, where the
    offspring had type 2 diabetes or impaired glucose homeostasis.  HSL
    promoter containing the HSL C-60G G-allele was generated and tested
    against a construct with the C-allele in HeLa cells and primary rat
    adipocytes.  HSL mRNA levels were quantified in subcutaneous and
    visceral fat from 33 obese subjects.Results: We found that the common
    C-allele was associated with increased waist circumference and WHR in lean
    controls, but there was no difference in genotype frequency between obese
    and non-obese subjects.  There was a significant increased transmission of
    C-alleles to the abdominally obese offspring but no increased transmission
    of C-alleles was observed to offspring with impaired glucose homeostasis.
    The G-allele showed reduced transcription in HeLa cells and primary rat
    adipocytes.  HSL mRNA levels were significantly higher in subcutaneous
    compared to visceral fat from obese subjects.Conclusion: The HSL C-60G
    polymorphism is associated with increased waist circumference in non-obese
    subjects.

CC

    Cytology - Animal   02506
    Cytology - Human   02508
    Genetics - General   03502
    Genetics - Animal   03506
    Genetics - Human   03508
    Biochemistry studies - Nucleic acids, purines and pyrimidines   10062
    Biochemistry studies - Lipids   10066
    Biochemistry studies - Carbohydrates   10068
    Pathology - General   12502
    Nutrition - General studies, nutritional status and methods   13202
    Nutrition - Malnutrition and obesity   13203

IT

    Major Concepts
       Molecular Genetics (Biochemistry and Molecular Biophysics); Nutrition;
       Human Medicine (Medical Sciences)

IT

    Parts, Structures, & Systems of Organisms
       adipocyte; subcutaneous fat; visceral fat

IT

    Diseases
       obesity: nutritional disease
       Obesity (MeSH)

IT

    Chemicals & Biochemicals
       triglycerides; mRNA [messenger RNA]; hormone-sensitive lipase;
       glucose: homeostasis

IT

    Miscellaneous Descriptors
       waist circumference; allele transmission

ORGN

    Classifier
       Hominidae   86215
    Super Taxa
       Primates; Mammalia; Vertebrata; Chordata; Animalia
    Organism Name
       HeLa cell line (cell_line)
       human (common): adult, middle age, female, male
    Taxa Notes
       Animals, Chordates, Humans, Mammals, Primates, Vertebrates

ORGN

    Classifier
       Muridae   86375
    Super Taxa
       Rodentia; Mammalia; Vertebrata; Chordata; Animalia
    Organism Name
       rat (common)
    Taxa Notes
       Animals, Chordates, Mammals, Nonhuman Vertebrates, Nonhuman Mammals,
       Rodents, Vertebrates

RN

    9001-62-1 (hormone-sensitive lipase)
    58367-01-4 (glucose)

GEN

    human HSL gene [human hormone-sensitive lipase gene] (Hominidae):
    promoter polymorphism, G-allele