AN
2003:317841 BIOSIS
DN
PREV200300317841
TI
Downregulation of islet hormone-sensitive lipase during long-term
high-fat feeding.
AU
Winzell, Maria Sorhede [Reprint Author]; Holm, Cecilia; Ahren, Bo
CS
Department of Cell and Molecular Biology, Sec. for Molecular Signalling,
Biomedical Center, Lund University, S-221 84, Lund, Sweden
Maria.Sorhede_Winzell@medkem.lu.se
SO
Biochemical and Biophysical Research Communications, (May 2 2003) Vol.
304, No. 2, pp. 273-278. print.
CODEN: BBRCA9. ISSN: 0006-291X.
DT
Article
LA
English
ED
Entered STN: 9 Jul 2003
Last Updated on STN: 9 Jul 2003
AB
Lipid accumulation in pancreatic beta-cells during high-fat (HF) feeding
may be involved in inducing a defective insulin secretion due to
lipotoxicity. Hormone-sensitive lipase (HSL) is expressed and
active in beta-cells, but its importance for islet dysfunction during the
development of type 2 diabetes is not known. In this study, prolonged
HF feeding of C57BL/6J mice, resulted in decreased HSL expression in
islets, representing only 25 +- 4% of the levels observed in controls.
This was paralleled by triglyceride accumulation and blunted insulin
secretion both in vivo and in vitro. After switching the HF diet to a LF
diet, HSL expression increased 10-fold compared to the HF fed mice.
This was accompanied by reduced triglyceride levels and a restored insulin
secretion. These results support the notion that HSL plays a critical
role in the regulation of intracellular triglyceride levels in beta-cells,
and that down-regulation of the enzyme may serve to protect against fatty
acid-induced islet dysfunction.
CC
Cytology - Animal 02506
Biochemistry studies - Proteins, peptides and amino acids 10064
Biochemistry studies - Lipids 10066
Enzymes - General and comparative studies: coenzymes 10802
Metabolism - Metabolic disorders 13020
Nutrition - General studies, nutritional status and methods 13202
Endocrine - General 17002
Endocrine - Pancreas 17008
Toxicology - General and methods 22501
IT
Major Concepts
Endocrine System (Chemical Coordination and Homeostasis); Enzymology
(Biochemistry and Molecular Biophysics); Nutrition
IT
Parts, Structures, & Systems of Organisms
pancreatic beta-cell: endocrine system
IT
Diseases
type 2 diabetes: endocrine disease/pancreas, metabolic disease
Diabetes Mellitus, Non-Insulin-Dependent (MeSH)
IT
Chemicals & Biochemicals
fat: nutrient; fatty acid; hormone-sensitive lipase:
downregulation; insulin; lipid: toxicity; triglyceride
ORGN
Classifier
Muridae 86375
Super Taxa
Rodentia; Mammalia; Vertebrata; Chordata; Animalia
Organism Name
mouse (common)
Taxa Notes
Animals, Chordates, Mammals, Nonhuman Vertebrates, Nonhuman Mammals,
Rodents, Vertebrates
RN
9001-62-1 (hormone-sensitive lipase)
9004-10-8 (insulin)