View source for DISSABS / 2006:175

AN   
     2006:175  DISSABS   Order Number: AAIC821247 (not available for sale by
     UMI)
TI   
     Hormone-sensitive lipase: New roles for an old enzyme
AU   
     Hansson, Ola Axel [Ph.D.]
CS   
     Lunds Universitet (Sweden) (0899)
SO   
     Dissertation Abstracts International, (2005) Vol. 66, No. 4C, p. 766.
     Order No.: AAIC821247 (not available for sale by UMI). 147 pages. 
     ISBN: 91-85439-76-2.
DT   
     Dissertation
FS   
     DAI
LA   
     English
ED   
     Entered STN: 20060126
     Last Updated on STN: 20060126
AB   
          Hormone-sensitive lipase (HSL) has a unique ability to
     hydrolyze a large panel of substrates including acylglycerols, cholesteryl
     esters and retinyl esters. HSL is potentially a new drug target for the
     treatment of obesity and type II diabetes. However, as HSL is not only
     expressed in white adipose tissue (WAT), but also plays significant roles
     in other tissues, it is of importance to better understand the different
     functions of this enzyme in the body.
          In this thesis the aim has been to study the consequences of a
     targeted disruption of the HSL gene in the mouse with focus on the
     effects in skeletal muscle and WAT.
          Expressional and functional analyses of soleus muscle of HSL null
     mice suggests an important role of HSL in skeletal muscle metabolism as
     the absence of HSL leads to increased glycogen utilization and an
     increased amount of lipid droplets, which presumably reflects a metabolic
     switch from lipid to carbohydrate metabolism.
          A fibre type transformation from slow twitch oxidative fibres to an
     enrichment of fast twitch glycolytic fibres in soleus muscle is also
     suggested.
          Using a proteomic approach a local inflammatory response in WAT is
     demonstrated in the non-obese HSL null mouse model. New methodological
     aspects of analysing data generated by two-dimensional polyacrylamide gel
     electrophoresis are also presented. Increased energy expenditure and
     perturbation of adipogenesis are suggested reasons behind the observed
     protection against diet-induced obesity in HSL null mice. Results
     presented in this thesis suggest an important role of HSL in lipid
     signalling and adipogenesis through its action as a retinyl ester
     hydrolyze in WAT.
CC   
     0379 BIOLOGY, CELL; 0487 CHEMISTRY, BIOCHEMISTRY; 0571 HEALTH SCIENCES,
     PATHOLOGY