View source for BIOSIS / 2006:632054

AN   
     2006:632054  BIOSIS
DN   
     PREV200600633817
TI   
     The hormone-sensitive lipase C-60G promoter polymorphism is
     associated with increased waist circumference in normal-weight subjects. 
AU   
     Carlsson, E. [Reprint Author]; Johansson, L. E.; Strom, K.; Hoffstedt, J.;
     Groop, L.; Holm, C.; Ridderstrale, M.
CS   
     Lund Univ, Malmo Univ Hosp, Dept Clin Sci Diabet and Endocrinol, S-20502
     Malmo, Sweden
     emma.carlsson@med.lu.se
SO   
     International Journal of Obesity, (SEP 2006) Vol. 30, No. 9, pp.
     1442-1448. 
     CODEN: IJOBDP. ISSN: 0307-0565. 
DT   
     Article
LA   
     English
ED   
     Entered STN: 22 Nov 2006
     Last Updated on STN: 22 Nov 2006
AB   
     Objective: Hormone-sensitive lipase (HSL) is a key enzyme in the
     mobilization of fatty acids from triglyceride stores in adipocytes.  The
     aim of the present study was to investigate the role of the HSL gene
     promoter variant C-60G, a polymorphism which previously has been
     associated with reduced promoter activity in vitro, in obesity and type 2
     diabetes.Design: We genotyped two materials consisting of obese subjects
     and non-obese controls, one material with offspring-parents trios, where
     the offspring was abdominally obese and one material with trios, where the
     offspring had type 2 diabetes or impaired glucose homeostasis.  HSL
     promoter containing the HSL C-60G G-allele was generated and tested
     against a construct with the C-allele in HeLa cells and primary rat
     adipocytes.  HSL mRNA levels were quantified in subcutaneous and
     visceral fat from 33 obese subjects.Results: We found that the common
     C-allele was associated with increased waist circumference and WHR in lean
     controls, but there was no difference in genotype frequency between obese
     and non-obese subjects.  There was a significant increased transmission of
     C-alleles to the abdominally obese offspring but no increased transmission
     of C-alleles was observed to offspring with impaired glucose homeostasis.
     The G-allele showed reduced transcription in HeLa cells and primary rat
     adipocytes.  HSL mRNA levels were significantly higher in subcutaneous
     compared to visceral fat from obese subjects.Conclusion: The HSL C-60G
     polymorphism is associated with increased waist circumference in non-obese
     subjects.
CC   
     Cytology - Animal   02506
     Cytology - Human   02508
     Genetics - General   03502
     Genetics - Animal   03506
     Genetics - Human   03508
     Biochemistry studies - Nucleic acids, purines and pyrimidines   10062
     Biochemistry studies - Lipids   10066
     Biochemistry studies - Carbohydrates   10068
     Pathology - General   12502
     Nutrition - General studies, nutritional status and methods   13202
     Nutrition - Malnutrition and obesity   13203
IT   
     Major Concepts
        Molecular Genetics (Biochemistry and Molecular Biophysics); Nutrition;
        Human Medicine (Medical Sciences)
IT   
     Parts, Structures, & Systems of Organisms
        adipocyte; subcutaneous fat; visceral fat
IT   
     Diseases
        obesity: nutritional disease
        Obesity (MeSH)
IT   
     Chemicals & Biochemicals
        triglycerides; mRNA [messenger RNA]; hormone-sensitive lipase;
        glucose: homeostasis
IT   
     Miscellaneous Descriptors
        waist circumference; allele transmission
ORGN 
     Classifier
        Hominidae   86215
     Super Taxa
        Primates; Mammalia; Vertebrata; Chordata; Animalia
     Organism Name
        HeLa cell line (cell_line)
        human (common): adult, middle age, female, male
     Taxa Notes
        Animals, Chordates, Humans, Mammals, Primates, Vertebrates
ORGN 
     Classifier
        Muridae   86375
     Super Taxa
        Rodentia; Mammalia; Vertebrata; Chordata; Animalia
     Organism Name
        rat (common)
     Taxa Notes
        Animals, Chordates, Mammals, Nonhuman Vertebrates, Nonhuman Mammals,
        Rodents, Vertebrates
RN   
     9001-62-1 (hormone-sensitive lipase)
     58367-01-4 (glucose)
GEN  
     human HSL gene [human hormone-sensitive lipase gene] (Hominidae):
     promoter polymorphism, G-allele