View source for BIOSIS / 2003:317841

AN   
     2003:317841  BIOSIS
DN   
     PREV200300317841
TI   
     Downregulation of islet hormone-sensitive lipase during long-term
     high-fat feeding. 
AU   
     Winzell, Maria Sorhede [Reprint Author]; Holm, Cecilia; Ahren, Bo
CS   
     Department of Cell and Molecular Biology, Sec. for Molecular Signalling,
     Biomedical Center, Lund University, S-221 84, Lund, Sweden
     Maria.Sorhede_Winzell@medkem.lu.se
SO   
     Biochemical and Biophysical Research Communications, (May 2 2003) Vol.
     304, No. 2, pp. 273-278. print. 
     CODEN: BBRCA9. ISSN: 0006-291X. 
DT   
     Article
LA   
     English
ED   
     Entered STN: 9 Jul 2003
     Last Updated on STN: 9 Jul 2003
AB   
     Lipid accumulation in pancreatic beta-cells during high-fat (HF) feeding
     may be involved in inducing a defective insulin secretion due to
     lipotoxicity.  Hormone-sensitive lipase (HSL) is expressed and
     active in beta-cells, but its importance for islet dysfunction during the
     development of type 2 diabetes is not known.  In this study, prolonged
     HF feeding of C57BL/6J mice, resulted in decreased HSL expression in
     islets, representing only 25 +- 4% of the levels observed in controls.
     This was paralleled by triglyceride accumulation and blunted insulin
     secretion both in vivo and in vitro.  After switching the HF diet to a LF
     diet, HSL expression increased 10-fold compared to the HF fed mice.
     This was accompanied by reduced triglyceride levels and a restored insulin
     secretion.  These results support the notion that HSL plays a critical
     role in the regulation of intracellular triglyceride levels in beta-cells,
     and that down-regulation of the enzyme may serve to protect against fatty
     acid-induced islet dysfunction.
CC   
     Cytology - Animal   02506
     Biochemistry studies - Proteins, peptides and amino acids   10064
     Biochemistry studies - Lipids   10066
     Enzymes - General and comparative studies: coenzymes   10802
     Metabolism - Metabolic disorders   13020
     Nutrition - General studies, nutritional status and methods   13202
     Endocrine - General   17002
     Endocrine - Pancreas   17008
     Toxicology - General and methods   22501
IT   
     Major Concepts
        Endocrine System (Chemical Coordination and Homeostasis); Enzymology
        (Biochemistry and Molecular Biophysics); Nutrition
IT   
     Parts, Structures, & Systems of Organisms
        pancreatic beta-cell: endocrine system
IT   
     Diseases
        type 2 diabetes: endocrine disease/pancreas, metabolic disease
        Diabetes Mellitus, Non-Insulin-Dependent (MeSH)
IT   
     Chemicals & Biochemicals
        fat: nutrient; fatty acid; hormone-sensitive lipase:
        downregulation; insulin; lipid: toxicity; triglyceride
ORGN 
     Classifier
        Muridae   86375
     Super Taxa
        Rodentia; Mammalia; Vertebrata; Chordata; Animalia
     Organism Name
        mouse (common)
     Taxa Notes
        Animals, Chordates, Mammals, Nonhuman Vertebrates, Nonhuman Mammals,
        Rodents, Vertebrates
RN   
     9001-62-1 (hormone-sensitive lipase)
     9004-10-8 (insulin)